Latest Posters

Study of an inducible lysis device in E. coli
(1,2)Susanna Zucca, (1,2) Lorenzo Pasotti, (2)Manuel Lupotto, (2)Maria Gabriella Cusella De Angelis, (1,2)Paolo Magni, (1)Dipartimento di Informatica e Sistemistica, Università degli Studi di Pavia, Italy; (2) Centro di Ingegneria Tissutale, Università degli Studi di Pavia, Italy,
In synthetic biological systems, cell lysis can be used to engineer programmed cell death and also to release proteins in extra-cellular environment, when secretion is not feasible. In this work, a promoterless lysis device called BBa_K112808, present in the Registry of Standard Biological Parts, was studied. A 3OC6-HSL inducible promoter was cloned upstream of the lysis device and used to characterize it.

Validation of a standard approach for quantitative characterization of promoter strength
Lorenzo Pasotti(1)(2), Susanna Zucca(1)(2), Maria Gabriella Cusella De Angelis(2), Paolo Magni(1)(2), (1)Dipartimento di Informatica e Sistemistica, Università degli Studi di Pavia, Italy ; (2)Centro di Ingegneria Tissutale, Università degli Studi di Pavia, Italy,
The rational design of synthetic biological systems can be performed only using a set of well-characterized biological parts. In this work, the Relative Promoter Units (RPUs) standard measurement method, proposed by Kelly et al., 2008, was validated and applied to characterize eight promoters from the Registry of Standard Biological Parts. The RPU approach allows the measurement of a promoter of interest using BBa_J23101 constitutive promoter as a standard reference.

Characterization of enamel and dentin protein expression in bioengineered human tooth tissues
Sheila Soroushian1, Monica Duailibi2, Silvio Duailibi2, Weibo Zhang1, Robert Litterman1, Ivy Pruit1, Pamela C. Yelick1, 1Department of Oral Biology, Division of Craniofacial and Molecular Genetics, Tufts University School of Dental Medicine, Boston MA 02111, and 2University Federal of São Paulo, Department of Plastic S,
Here we have used previously described methods to bioengineer teeth from human dental stem cells (hDSC). We we were able to demonstrate that dissociated hDSC-seeded polymer constructs can form bioengineered teeth that are morphologically similar to natural human teeth at both gross and microscopic levels. We anticipate that further modifications of this approach will eventually result in reliable methods to bioengineer replacement teeth in humans.

Aspects/conflicts of marine resource use – Segara Anakan – Central Java – Indonnesia
Christian Reichel, Centre of Marine Ecology (ZMT) Bremen/ Institute for Social Anthropology Freie Universität Berlin ,
Cultural skills that could lead to a reduction in vulnerability should be explored. This especially includes the sustainable handling of endangered nature and cultural areas as well as the prevention and treatment of catastrophes.

Study of volumetric oxygen transfer coefficient in a Taylor vortex flow bioreactor
Patricia Ap. Santiago; Claudio A Suazo; Roberto C Giordano., Department of Chemical Engineering, Federal University of Sao Carlos, P.O. Box 676, Post Code 13565-905, Sao Carlos, SP, Brazil.,
In biochemical processes involving cultivation of animal cells, the supply of oxygen in culture medium is an extremely important parameter in the project of bioreactors and its operation. In this work, the global volumetric transfer coefficient of oxygen (KLa) is evaluated in a Taylor vortex flow bioreactor (TVFB) and a correlation of mass transfer, of this equipment, is proposed.

Nonlinear Dynamic Models for Single-Cell Time-Lapse Microscopy
Jarad Niemi, Cheemeng Tan, Lingchong You, Mike West, Duke University,
This poster applies Bayesian analysis of nonlinear dynamic models to time-lapse fluorescent microscopy data.