Latest Posters

Digital Volumetric Imaging of Angiogenesis in Tissue Engineered Constructs
Marc M. Takeno, Kip D. Hauch, University of Washington,
Accurate identification of functional vasculature is of critical importance in evaluating construct integration. Digital volumetric imaging (DVI) is an automated serial sectioning and microscopic imaging technique that can bridge the resolution gap between micro-computed tomography and conventional histology. We use DVI to study in vivo vascularization of tissue engineering constructs in a variety of settings.

Study of an inducible lysis device in E. coli
(1,2)Susanna Zucca, (1,2) Lorenzo Pasotti, (2)Manuel Lupotto, (2)Maria Gabriella Cusella De Angelis, (1,2)Paolo Magni, (1)Dipartimento di Informatica e Sistemistica, Università degli Studi di Pavia, Italy; (2) Centro di Ingegneria Tissutale, Università degli Studi di Pavia, Italy,
In synthetic biological systems, cell lysis can be used to engineer programmed cell death and also to release proteins in extra-cellular environment, when secretion is not feasible. In this work, a promoterless lysis device called BBa_K112808, present in the Registry of Standard Biological Parts, was studied. A 3OC6-HSL inducible promoter was cloned upstream of the lysis device and used to characterize it.

Validation of a standard approach for quantitative characterization of promoter strength
Lorenzo Pasotti(1)(2), Susanna Zucca(1)(2), Maria Gabriella Cusella De Angelis(2), Paolo Magni(1)(2), (1)Dipartimento di Informatica e Sistemistica, Università degli Studi di Pavia, Italy ; (2)Centro di Ingegneria Tissutale, Università degli Studi di Pavia, Italy,
The rational design of synthetic biological systems can be performed only using a set of well-characterized biological parts. In this work, the Relative Promoter Units (RPUs) standard measurement method, proposed by Kelly et al., 2008, was validated and applied to characterize eight promoters from the Registry of Standard Biological Parts. The RPU approach allows the measurement of a promoter of interest using BBa_J23101 constitutive promoter as a standard reference.

Characterization of enamel and dentin protein expression in bioengineered human tooth tissues
Sheila Soroushian1, Monica Duailibi2, Silvio Duailibi2, Weibo Zhang1, Robert Litterman1, Ivy Pruit1, Pamela C. Yelick1, 1Department of Oral Biology, Division of Craniofacial and Molecular Genetics, Tufts University School of Dental Medicine, Boston MA 02111, and 2University Federal of São Paulo, Department of Plastic S,
Here we have used previously described methods to bioengineer teeth from human dental stem cells (hDSC). We we were able to demonstrate that dissociated hDSC-seeded polymer constructs can form bioengineered teeth that are morphologically similar to natural human teeth at both gross and microscopic levels. We anticipate that further modifications of this approach will eventually result in reliable methods to bioengineer replacement teeth in humans.