Validation of a standard approach for quantitative characterization of promoter strength
Lorenzo Pasotti(1)(2), Susanna Zucca(1)(2), Maria Gabriella Cusella De Angelis(2), Paolo Magni(1)(2),
(1)Dipartimento di Informatica e Sistemistica, Università degli Studi di Pavia, Italy ; (2)Centro di Ingegneria Tissutale, Università degli Studi di Pavia, Italy
Date Posted: Sunday, February 28, 2010
The rational design of synthetic biological systems can be performed only using a set of well-characterized biological parts. In this work, the Relative Promoter Units (RPUs) standard measurement method, proposed by Kelly et al., 2008, was validated and applied to characterize eight promoters from the Registry of Standard Biological Parts. The RPU approach allows the measurement of a promoter of interest using BBa_J23101 constitutive promoter as a standard reference.
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